Coding

Part:BBa_M10053:Design

Designed by: Susan Chen   Group: Berkeley BioE140L - S09   (2009-05-06)

{˂EILD˃}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Synthetic 

Wobble Osc0028F/Osc0029R           (136bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5      (EcoRI/BamHI, 3171+910, L)
Product is pBca9495AK-M10053    {<EILD>}
----
Osc0028F   Forward construction of Leucine zipper EILD
TCCGGgaattcATGagatctGTTAAAGAACTGGAAGACAAAAACGAAGAACTGCTGTCTGAAATCTACCACCTGGACAACGAAG
Osc0029R   Reverse construction of Leucine zipper EILD
CTGGTggatccGCAACCACCACGTTCACCAACCAGTTTTTTCAGACGAGCAACTTCGTTGTCCAGGTGGTAG

References

Havranek, James J. and Pehr B. Harbury. (2002) Automated design of specificity in molecular recognition. Nature structural biology 10: 45-52.